Difference between revisions of "Part:BBa K3429002"
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Flourescence Protein optimized for B. subtilis codon usage.
 
Flourescence Protein optimized for B. subtilis codon usage.
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We use this <b>flexible glycine-serine linker</b> (GGGGSGGGGS) to create TasA fusion proteins with CotA and CuO. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function.
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<a class="anchor" id="cite_note-1"></a>
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            <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:04, 25 October 2020


Superfolder Green Fluorescent Protein (sfGFP)

Flourescence Protein optimized for B. subtilis codon usage.
We use this flexible glycine-serine linker (GGGGSGGGGS) to create TasA fusion proteins with CotA and CuO. Huang et al fused TasA to superfolder GFP by using this protein linker without impairing TasA or sfGFP function.



<a class="anchor" id="cite_note-1"></a> 

           <a class="referencestd" href="https://doi.org/10.1038/s41589-018-0169-2 " target="_blank">7. Huang, J., Liu, S., Zhang, C. et al. Programmable and printable Bacillus subtilis biofilms as engineered living materials. Nat Chem Biol 15, 34–41 (2019) </a>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]