Difference between revisions of "Part:BBa K3606008"

 
Line 3: Line 3:
 
<partinfo>BBa_K3606008 short</partinfo>
 
<partinfo>BBa_K3606008 short</partinfo>
  
~
+
This promoter comes from a kit which contains combinations of specific constitutive bacterial promoters that vary in strength.
 +
 
 +
<h2>Design:</h2>
 +
We have chosen a series of constitutive promoters with different strength which include P1. They are used to reliably drive expression of CaAP and McbABCEFG in E. coli in a controlled, reliable manner. We have tested these different, unique combinations to find the best expression level of CaAP and McbABCEFG.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:08, 25 October 2020


P1 promoter

This promoter comes from a kit which contains combinations of specific constitutive bacterial promoters that vary in strength.

Design:

We have chosen a series of constitutive promoters with different strength which include P1. They are used to reliably drive expression of CaAP and McbABCEFG in E. coli in a controlled, reliable manner. We have tested these different, unique combinations to find the best expression level of CaAP and McbABCEFG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]