Difference between revisions of "Part:BBa K3606008"
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<partinfo>BBa_K3606008 short</partinfo> | <partinfo>BBa_K3606008 short</partinfo> | ||
− | + | This promoter comes from a kit which contains combinations of specific constitutive bacterial promoters that vary in strength. | |
+ | |||
+ | <h2>Design:</h2> | ||
+ | We have chosen a series of constitutive promoters with different strength which include P1. They are used to reliably drive expression of CaAP and McbABCEFG in E. coli in a controlled, reliable manner. We have tested these different, unique combinations to find the best expression level of CaAP and McbABCEFG. | ||
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Revision as of 05:08, 25 October 2020
P1 promoter
This promoter comes from a kit which contains combinations of specific constitutive bacterial promoters that vary in strength.
Design:
We have chosen a series of constitutive promoters with different strength which include P1. They are used to reliably drive expression of CaAP and McbABCEFG in E. coli in a controlled, reliable manner. We have tested these different, unique combinations to find the best expression level of CaAP and McbABCEFG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]