Difference between revisions of "Part:BBa K3332080"
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It can express cI repressor under certain conditions.By using this part, we can make engineering bacteria survive at low formaldehyde concentration if we want. | It can express cI repressor under certain conditions.By using this part, we can make engineering bacteria survive at low formaldehyde concentration if we want. | ||
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The promoter BAD/araC is induced under arabinose and cI repressor is the co-protein of MazF, reduces the toxicity of MazF after their combination, provides the minimum | ||
| + | margin of formaldehyde-induced cell toxicity. | ||
| + | [[File:T--XMU-2080.circuit.png|none|500px|caption]] | ||
| + | Fig1. Circuit | ||
| + | |||
| + | ===Characterization=== | ||
| + | When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid is correct. We used the ''EcoR'' I and ''Pst'' I to cut the plasmid, then we got the target separate fragment-2148bp | ||
| + | |||
| + | [[File:T-XMU-BBa K3332080.ger.png|none|500px|caption]] | ||
| + | Fig.2 I0500_B0034_cI_B0015_pSB1C3[BBa_K3332080] digested by ''EcoR'' I and ''Pst'' I | ||
| + | |||
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Revision as of 03:36, 25 October 2020
pBAD/araC promoter-cI repressor-terminator
It can express cI repressor under certain conditions.By using this part, we can make engineering bacteria survive at low formaldehyde concentration if we want.
Usage and Biology
The promoter BAD/araC is induced under arabinose and cI repressor is the co-protein of MazF, reduces the toxicity of MazF after their combination, provides the minimum margin of formaldehyde-induced cell toxicity.
Fig1. Circuit
Characterization
When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit was built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid is correct. We used the EcoR I and Pst I to cut the plasmid, then we got the target separate fragment-2148bp
Fig.2 I0500_B0034_cI_B0015_pSB1C3[BBa_K3332080] digested by EcoR I and Pst I
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961