Difference between revisions of "Part:BBa K3365006"

 
Line 3: Line 3:
 
<partinfo>BBa_K3365006 short</partinfo>
 
<partinfo>BBa_K3365006 short</partinfo>
  
−
target sequence is part of the PDCD1 gene
+
The PAM and target sequence is located directly downstream of the promoter, where dCas9 could bind and block RNAP. In our part, the “target” is the target sequence for PDCD1 CRISPR gene editing in one clinical trial, which can be identified and bound by the complex of dCas9 and corresponding sgRNA.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:33, 25 October 2020


Target sequence downstream of pBAD/araC

The PAM and target sequence is located directly downstream of the promoter, where dCas9 could bind and block RNAP. In our part, the “target” is the target sequence for PDCD1 CRISPR gene editing in one clinical trial, which can be identified and bound by the complex of dCas9 and corresponding sgRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961