Difference between revisions of "Part:BBa K117000:Design"

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===Design Notes===
 
===Design Notes===
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Lysis gene was commercially synthesized by ****.  
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Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]
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==Extraction of Lysis gene/PCR Oligo fragments DNA sequence
+
 
 +
Extraction of Lysis gene/PCR Oligo fragments DNA sequence
 
Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
 
Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
  
 
==Source of Orgin sequence==
 
==Source of Orgin sequence==
−
to be updated.
+
to be updated
  
 
===Source===
 
===Source===
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===References===
 
===References===
 +
Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100
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 +
Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 "

Latest revision as of 16:25, 29 October 2008

Lysis gene (promotes lysis in colicin-producing bacteria strain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lysis gene was commercially synthesized by [http://www.sciencewerke.com Science Wereke]

Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Source of Orgin sequence

to be updated

Source

to be updated

References

Cloning and Characterization of ColE7 Plasmid. J Gen Microbiol 137:97-100

Characterization of the cea gene of the ColE7 Plasmid. Mol Gen Genet (1992) 233:177-183 "