Difference between revisions of "Part:BBa K3332038"
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pTrc-2 promoter is used to express mf-lon and MazF in the absence of aTc so as to inhibit the growth of E.coli. It is part of the circut designed to prevent engineered bacteria in the detection instrument from escaping. | pTrc-2 promoter is used to express mf-lon and MazF in the absence of aTc so as to inhibit the growth of E.coli. It is part of the circut designed to prevent engineered bacteria in the detection instrument from escaping. | ||
In this circuit, LacI can repress ptrc-2 promoter and ptrc-2 derived promoter while the LacI can repress the pLtetO-1 promoter. When the aTc exits, it can combine tetR, so that the pLtetO-1 promoter can’t be repressed. Then the LacI which is controlled by the pLtetO-1 can repress the ptrc-2 promoter and ptrc-2 derived promoter. As a result, mf-lon and mazF can’t be expressed. As a kind of bacterial toxin, mazF can cause the bacteria death. So there comes the conclusion that as long as the engineered E.coli are cultured in the environment with aTc, it won’t be killed by the mazF, but when the bacteria escape from our testing instrument, the effect can be reversed, that is to say, the bacteria will be killed by the mazF. In the same way, we can conclude that in the presence of IPTG, MazF can be expressed to cause bacterial death. | In this circuit, LacI can repress ptrc-2 promoter and ptrc-2 derived promoter while the LacI can repress the pLtetO-1 promoter. When the aTc exits, it can combine tetR, so that the pLtetO-1 promoter can’t be repressed. Then the LacI which is controlled by the pLtetO-1 can repress the ptrc-2 promoter and ptrc-2 derived promoter. As a result, mf-lon and mazF can’t be expressed. As a kind of bacterial toxin, mazF can cause the bacteria death. So there comes the conclusion that as long as the engineered E.coli are cultured in the environment with aTc, it won’t be killed by the mazF, but when the bacteria escape from our testing instrument, the effect can be reversed, that is to say, the bacteria will be killed by the mazF. In the same way, we can conclude that in the presence of IPTG, MazF can be expressed to cause bacterial death. | ||
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<table><tr><th>[[File:T--XMU-China--BBa_K3332086.png|thumb|300px|Fig.2 pTrc-2_E0420_pSB1C3[BBa_K3332086] digested by <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table> | <table><tr><th>[[File:T--XMU-China--BBa_K3332086.png|thumb|300px|Fig.2 pTrc-2_E0420_pSB1C3[BBa_K3332086] digested by <i>Xba</i>I and <i>Pst</i>I.]]</th><th></table> | ||
| − | + | ===Sequence and Features=== | |
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<partinfo>BBa_K3332038 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3332038 SequenceAndFeatures</partinfo> | ||
Revision as of 02:28, 25 October 2020
pTrc-2
A promoter derived from pTrc-2 promoter can be strongly repressed by LacI protein. Ptrc-2 promoter has one lac operator, which means that it has less LacI binding sites so that LacI has a weak inhibitory effect on it.
Usage and Biology
pTrc-2 promoter is used to express mf-lon and MazF in the absence of aTc so as to inhibit the growth of E.coli. It is part of the circut designed to prevent engineered bacteria in the detection instrument from escaping. In this circuit, LacI can repress ptrc-2 promoter and ptrc-2 derived promoter while the LacI can repress the pLtetO-1 promoter. When the aTc exits, it can combine tetR, so that the pLtetO-1 promoter can’t be repressed. Then the LacI which is controlled by the pLtetO-1 can repress the ptrc-2 promoter and ptrc-2 derived promoter. As a result, mf-lon and mazF can’t be expressed. As a kind of bacterial toxin, mazF can cause the bacteria death. So there comes the conclusion that as long as the engineered E.coli are cultured in the environment with aTc, it won’t be killed by the mazF, but when the bacteria escape from our testing instrument, the effect can be reversed, that is to say, the bacteria will be killed by the mazF. In the same way, we can conclude that in the presence of IPTG, MazF can be expressed to cause bacterial death.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]