Difference between revisions of "Part:BBa K3440014"
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===Characterization=== | ===Characterization=== | ||
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. | ||
| − | + | We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part. | |
[[File:BBa_K3440000_WB.png|thumb|center|500px|Western blot with Q3 as BBa_K3440014]] | [[File:BBa_K3440000_WB.png|thumb|center|500px|Western blot with Q3 as BBa_K3440014]] | ||
[[File:T--Stockholm--greenfluo.png|thumb|center|500px|Fluorescent measurements with Q3 as BBa_K3440014]] | [[File:T--Stockholm--greenfluo.png|thumb|center|500px|Fluorescent measurements with Q3 as BBa_K3440014]] | ||
Revision as of 00:30, 25 October 2020
GFP under Plux in presence of LuxR and AHL
Pconst(BBa_J23100)-RBS(BBa_B0034)-LuxR(BBa_C0062)-Myc(BBa_K823036)-DT(BBa_B0015)-Plux(BBa_B0015)-RBS(BBa_B0034)-GFP(BBa_E0040)
Usage and Biology
This part produces GFP in the presence of 3OC6-HSL. 3OC6-HSL can from a complex with LuxR, which is expressed constitutively. the complex can activate Plux promoter, under which GFP is placed.
Characterization
Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part. We sent this part for sequencing to Microsynth AG. The sequence obtained corresponded to the expected part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 874
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1023
Illegal BsaI.rc site found at 1750