Difference between revisions of "Part:BBa K3466002:Design"
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===Source=== | ===Source=== | ||
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| + | ttgacggctagctcagtcctaggtacagtgctagcgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggcaccggttgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgaccggtatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaataaagaggagaaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata | ||
===References=== | ===References=== | ||
Revision as of 22:03, 24 October 2020
CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 293
Illegal AgeI site found at 395
Illegal AgeI site found at 1168
Illegal AgeI site found at 1280 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used a spacer to test whether or not it serves to enhance our RFP expression rate.
Source
ttgacggctagctcagtcctaggtacagtgctagcgaaagaggggacaaactagaatggagaacaatctggagaacctgaccattggcgtgtttgccaaagccgccggtgtgaacgtggaaaccatccgcttctatcagcgcaaaggtctgctgcgcgaaccggataaaccgtatggcagcattcgccgctatggtgaagccgatgtggtgcgcgtgaaattcgtgaaaagcgcccagcgtctgggctttagcctggacgagattgccgactttctgcgcattgacgaaggcaccggttgtgtgggtcatgacgccctggccgaacataaactgaaggatgtgcgcgagaaaatggccgacctggcccgcatggaaaccgtgctgagcgagctgaccggtatgtgtaatctgccgccgggcaatgtgagctgtccgctgattgaaagcttacaaggtgaagcaggtctggcccgtagcgcaatgccgtaattgactctgtagttgctacagggtgtgcaataaagaggagaaatccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata