Difference between revisions of "Part:BBa K3338000"
Jonas Scholz (Talk | contribs) |
Jonas Scholz (Talk | contribs) |
||
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3338000 short</partinfo> | <partinfo>BBa_K3338000 short</partinfo> | ||
| + | |||
| + | ===Usage and Biology=== | ||
MagA is a transmembrane iron transporter originating from the aquatic magnetotactic bacterium <i>Magnetospirillum magnetotacticum</i>. In the original host the protein is involved in the formation of intracellular magnetic particles of magnetite (Fe<sub>3</sub>O<sub>4</sub>) (Nakamura <i>et al.</i> 1995, Uebe <i>et al.</i> 2012). These particles are the core components of magnetosomes in aquatic magnetotactic bacteria allowing them to passively align and swim along the earth's magnetic field lines (Lower and Bazylinski 2013). When expressed in procaryotic or eukaryotic cells it leads to the accumulation of intracellular iron (Goldhawk <i>et al.</i> 2009, Zurkiya <i>et al.</i> 2008). In the last years its capability as a reporter gene in mammalian cells using the non-invasive imaging technique magnetic resonance imaging (MRI) was recognized (Goldhawk <i>et al.</i> 2009, Zurkiya <i>et al.</i> 2008). It was shown that cells expressing MagA that are transplanted in animals can be detected using MRI without being incubated with iron supplement prior to transplantation (Zurkiya <i>et al.</i> 2008, Rohani 2014). However, not all cells types are suitable for MagA expression because it sometimes induces strong cytotoxic effects (Pereira 2016). In the HeLa cell system, we used in our study, MagA was properly expressed. | MagA is a transmembrane iron transporter originating from the aquatic magnetotactic bacterium <i>Magnetospirillum magnetotacticum</i>. In the original host the protein is involved in the formation of intracellular magnetic particles of magnetite (Fe<sub>3</sub>O<sub>4</sub>) (Nakamura <i>et al.</i> 1995, Uebe <i>et al.</i> 2012). These particles are the core components of magnetosomes in aquatic magnetotactic bacteria allowing them to passively align and swim along the earth's magnetic field lines (Lower and Bazylinski 2013). When expressed in procaryotic or eukaryotic cells it leads to the accumulation of intracellular iron (Goldhawk <i>et al.</i> 2009, Zurkiya <i>et al.</i> 2008). In the last years its capability as a reporter gene in mammalian cells using the non-invasive imaging technique magnetic resonance imaging (MRI) was recognized (Goldhawk <i>et al.</i> 2009, Zurkiya <i>et al.</i> 2008). It was shown that cells expressing MagA that are transplanted in animals can be detected using MRI without being incubated with iron supplement prior to transplantation (Zurkiya <i>et al.</i> 2008, Rohani 2014). However, not all cells types are suitable for MagA expression because it sometimes induces strong cytotoxic effects (Pereira 2016). In the HeLa cell system, we used in our study, MagA was properly expressed. | ||
| Line 9: | Line 11: | ||
</html> | </html> | ||
| − | References: | + | |
| + | ===Sequence and Features=== | ||
| + | |||
| + | <partinfo>BBa_K3338000 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | |||
| + | |||
| + | =Characterization= | ||
| + | |||
| + | |||
| + | |||
| + | =References:= | ||
<ol> | <ol> | ||
<li> | <li> | ||
| Line 33: | Line 46: | ||
</li> | </li> | ||
</ol> | </ol> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
Revision as of 20:57, 24 October 2020
Iron transporter MagA from Magnetospirillum magneticum
Usage and Biology
MagA is a transmembrane iron transporter originating from the aquatic magnetotactic bacterium Magnetospirillum magnetotacticum. In the original host the protein is involved in the formation of intracellular magnetic particles of magnetite (Fe3O4) (Nakamura et al. 1995, Uebe et al. 2012). These particles are the core components of magnetosomes in aquatic magnetotactic bacteria allowing them to passively align and swim along the earth's magnetic field lines (Lower and Bazylinski 2013). When expressed in procaryotic or eukaryotic cells it leads to the accumulation of intracellular iron (Goldhawk et al. 2009, Zurkiya et al. 2008). In the last years its capability as a reporter gene in mammalian cells using the non-invasive imaging technique magnetic resonance imaging (MRI) was recognized (Goldhawk et al. 2009, Zurkiya et al. 2008). It was shown that cells expressing MagA that are transplanted in animals can be detected using MRI without being incubated with iron supplement prior to transplantation (Zurkiya et al. 2008, Rohani 2014). However, not all cells types are suitable for MagA expression because it sometimes induces strong cytotoxic effects (Pereira 2016). In the HeLa cell system, we used in our study, MagA was properly expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1182
Illegal BsaI.rc site found at 514
Illegal BsaI.rc site found at 1063
Illegal SapI site found at 204
Characterization
References:
- Nakamura, C., Burgess, J. G., Sode, K., & Matsunaga, T. (1995). An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1. The Journal of biological chemistry, 270(47), 28392–28396.
- Uebe, R., Henn, V., & Schüler, D. (2012). The MagA protein of Magnetospirilla is not involved in bacterial magnetite biomineralization. Journal of bacteriology, 194(5), 1018–1023.
- Lower, B. H., & Bazylinski, D. A. (2013). The bacterial magnetosome: a unique prokaryotic organelle. Journal of molecular microbiology and biotechnology, 23(1-2), 63–80.
- Goldhawk, D. E., Lemaire, C., McCreary, C. R., McGirr, R., Dhanvantari, S., Thompson, R. T., Figueredo, R., Koropatnick, J., Foster, P., & Prato, F. S. (2009). Magnetic resonance imaging of cells overexpressing MagA, an endogenous contrast agent for live cell imaging. Molecular imaging, 8(3), 129–139.
- Zurkiya, O., Chan, A. W., & Hu, X. (2008). MagA is sufficient for producing magnetic nanoparticles in mammalian cells, making it an MRI reporter. Magnetic resonance in medicine, 59(6), 1225–1231.
- Rohani, R., Figueredo, R., Bureau, Y., Koropatnick, J., Foster, P., Thompson, R. T., Prato, F. S., & Goldhawk, D. E. (2014). Imaging tumor growth non-invasively using expression of MagA or modified ferritin subunits to augment intracellular contrast for repetitive MRI. Molecular imaging and biology, 16(1), 63–73.
- Pereira, S. M., Williams, S. R., Murray, P., & Taylor, A. (2016). MS-1 magA: Revisiting Its Efficacy as a Reporter Gene for MRI. Molecular imaging, 15, 1536012116641533.