Difference between revisions of "Part:BBa K1362424:Experience"
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===Applications of BBa_K1362424=== | ===Applications of BBa_K1362424=== | ||
| + | *Team:iGEM-IISERPUNE-2020 | ||
| + | *We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 18:23, 24 October 2020
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1362424
- Team:iGEM-IISERPUNE-2020
- We aimed to use this part for circularization of the cyclotide protein. For using this we found that it had 7 nucleotide sequences that made it incompatible for Gibson's assembly/Restriction free cloning. The 7 length sequence makes it out of frame with other components of the composite structure. So without disturbing the restriction site we designed a similar part that overcomes this problem: BBa_K3582021.
User Reviews
UNIQf55fab69e38cbed1-partinfo-00000000-QINU UNIQf55fab69e38cbed1-partinfo-00000001-QINU