Difference between revisions of "Part:BBa K3505018"
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[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
 
[[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
  
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[[File:T--Thessaly--sfpiato.jpeg|1200px|thumb|none|<i><b>Fig.3:</b>sfGFP uder control of pTRC</i>]]
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[[File:T--Thessaly--sfpiato.jpeg|700px|thumb|none|<i><b>Fig.3:</b>sfGFP uder control of pTRC</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 16:12, 24 October 2020


sfGFP GB compatible with B3-B5 Level 0 sfGFP

Fig.1:sfGFP
Fig.2:The overhangs of this part in the Golden Braid Grammar
Fig.3:sfGFP uder control of pTRC

Usage and Biology

A reporter gene fluorescent protein

  • Excitation at 485 nm
  • Emission at 510 nm


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Verification of cloning

Fig.4:U4 C4 Level0 sfGFP Digested with EcoRI, PstI Expected bands 2029, 794

Experimental Use and Experience

This part is used in BBa_K3505030

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]