Difference between revisions of "Part:BBa K3505018"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3505018 short</partinfo> | <partinfo>BBa_K3505018 short</partinfo> | ||
| + | Level 0 sfGFP | ||
| + | [[File:T--Thessaly--sf.png|700px|thumb|none|<i><b>Fig.1:</b>sfGFP</i>]] | ||
| − | [[File:T--Thessaly--GB-AATG-GCTT.jpeg| | + | [[File:T--Thessaly--GB-AATG-GCTT.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]] |
| − | [[File:T--Thessaly--sfpiato.png|600px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--sfpiato.png|600px|thumb|none|<i><b>Fig.3:</b>sfGFP uder control of pTRC</i>]] |
===Usage and Biology=== | ===Usage and Biology=== | ||
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===Verification of cloning=== | ===Verification of cloning=== | ||
| − | [[File:T--Thessaly--sf0.png|600px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--sf0.png|600px|thumb|none|<i><b>Fig.4:</b>U4 C4 Level0 sfGFP Digested with EcoRI, PstI Expected bands 2029, 794</i>]] |
===Experimental Use and Experience=== | ===Experimental Use and Experience=== | ||
Revision as of 15:37, 24 October 2020
sfGFP GB compatible with B3-B5
Level 0 sfGFP
File:T--Thessaly--sfpiato.png
Fig.3:sfGFP uder control of pTRC
Usage and Biology
A reporter gene fluorescent protein
- Excitation at 485 nm
- Emission at 510 nm
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.
Verification of cloning
Experimental Use and Experience
This part is used in BBa_K3505030
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 149
- 1000COMPATIBLE WITH RFC[1000]