Difference between revisions of "Part:BBa K3402005"
 
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<partinfo>BBa_K3402005 short</partinfo>
 
<partinfo>BBa_K3402005 short</partinfo>
  
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Peno is the promoter of enolase from S. bombicola.
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P<i>eno</i> is the promoter of enolase from <i>Starmerella bombicola</i>.
  
 
===Usage and Biology===
 
===Usage and Biology===
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We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
 
We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
  
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[[Image:Expression of Peno by yeGFP.png|700px|thumb|center|Expression of P<i>eno</i> by <i>yeGFP</i>]]
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[[Image:Expression of Peno by yeGFP.png|700px|thumb|center|'''Left:''' Expression of P<i>eno</i> by <i>yeGFP</i>, '''Middle:''' Fluorescence intensity, '''Right:''' Transcription level]]
  
 
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Latest revision as of 15:17, 24 October 2020


Peno

Peno is the promoter of enolase from Starmerella bombicola.

Usage and Biology

We used green fluorescence protein yeGFP(BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Peno by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 475
    Illegal XhoI site found at 1189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90