Difference between revisions of "Part:BBa K3402002"
 
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We used green fluorescence protein <i>yeGFP</i> ([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
 
We used green fluorescence protein <i>yeGFP</i> ([https://parts.igem.org/Part:BBa_K3402000# BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>.
  
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[[Image:Expression of Ppgi by yeGFP.png|700px|thumb|center|Expression of P<i>pgi</i> by <i>yeGFP</i>]]
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[[Image:Expression of Ppgi by yeGFP.png|700px|thumb|center|'''Left:''' Expression of P<i>pgi</i> by <i>yeGFP</i>, '''Middle:''' Fluorescence intensity, '''Right:''' Transcription level]]
  
 
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Latest revision as of 15:14, 24 October 2020


Ppgi

Ppgi is the promoter of glucose-6-phosphate isomerase from Starmerella bombicola.

Usage and Biology

We used green fluorescence protein yeGFP (BBa_K3402000) to characterize the strength of this promoter in S. bombicola.

Left: Expression of Ppgi by yeGFP, Middle: Fluorescence intensity, Right: Transcription level

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 259
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 259
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 259
    Illegal BglII site found at 837
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 259
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 259
  • 1000
    COMPATIBLE WITH RFC[1000]