Difference between revisions of "Part:BBa K2804002"

 
(4 intermediate revisions by the same user not shown)
Line 5: Line 5:
 
The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.
 
The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.
  
==Added by KEYSTONE_A 2020==
+
=='''Characterization of BMP2's effects in cartilage regeneration (by KEYSTONE_A 2020)'''==
 +
 
 +
BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro '''(Figure 3)'''. Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only '''(Figure 4)'''. For in vivo experiments, cotransplantation with BMP2 + sVEGFR1 can also promote cartilage formation '''(Figure 5)'''.
  
BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro.
 
  
[[File:KEYSTONE A BMP2 VEGFR effect.jpg|600px|thumb|left|'''Figure: SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.''']]
 
  
 
'''Reference'''
 
'''Reference'''
Line 15: Line 15:
 
Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.
 
Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.
  
 +
[[File:KEYSTONE A BMP2 VEGFR effect.jpg|600px|thumb|left|'''Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.''']]
  
<!-- Add more about the biology of this part here
+
[[File:KEYSTONE A BMP2 VEGFR effect_2.jpg|600px|thumb|left|'''Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1''']]
===Usage and Biology===
+
  
==Added by KEYSTONE_A 2020==
+
[[File:KEYSTONE A BMP2 VEGFR effect_in vivo.jpg|600px|thumb|left|'''Figure 5. Cotransplantation with BMP2 + sVEGFR1 led to increased cartilage formation in vivo''']]
 
+
BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro.
+
 
+
[[File:KEYSTONE A BMP2 VEGFR effect.jpg|600px|thumb|left|'''Figure: SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.''']]
+
 
+
'''Reference'''
+
 
+
Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.
+
  
  

Latest revision as of 14:57, 24 October 2020


Bone morphogenetic protein 2 (BMP2)

The human bone morphogenetic protein 2 is a bone-growth regulatory factor well known to be a potent osteoinductive protein.

Characterization of BMP2's effects in cartilage regeneration (by KEYSTONE_A 2020)

BMP2 and VEGF inhibition promote expression of cartilaginous pathways on sorted skeletal stem-cell (SSC) in vitro (Figure 3). Skeletal stem-cells (SSCs) isolated from the articular surface, periosteum and bone marrow all produced higher amounts of cartilage with addition of BMP2 + sVEGFR1 when compared with addition of PBS only (Figure 4). For in vivo experiments, cotransplantation with BMP2 + sVEGFR1 can also promote cartilage formation (Figure 5).


Reference

Murphy, M. P., Koepke, L. S., Lopez, M. T., Tong, X., Ambrosi, T. H., Gulati, G. S., ... & Steininger, H. (2020). Articular cartilage regeneration by activated skeletal stem cells. Nature Medicine, 1-10.

Figure 3. SSC gene expression levels after in vitro treatment of PBS, BMP2, or sVEGFR1.
Figure 4. Alcian blue staining (for acidic glycans in cartilage) of SSC following chondrogenic differentiation with treatment of PBS or BMP2 + sVEGFR1
Figure 5. Cotransplantation with BMP2 + sVEGFR1 led to increased cartilage formation in vivo


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]