Difference between revisions of "Part:BBa K3506009:Design"
Line 15: Line 15:
 
CLB2 promoter is from the genome of Saccharomyces cerevisiae BY4741.
 
CLB2 promoter is from the genome of Saccharomyces cerevisiae BY4741.
  
−
Cas9 is from the PRH003 plasmid.  
+
Cas9 is from the pRH003 plasmid.  
  
  
 
===References===
 
===References===

Revision as of 11:46, 24 October 2020


The cassette of periodic expression of Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 812
    Illegal BglII site found at 1607
    Illegal BamHI site found at 1901
    Illegal XhoI site found at 203
    Illegal XhoI site found at 2407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 730
    Illegal BsaI.rc site found at 1972
    Illegal BsaI.rc site found at 3328
    Illegal BsaI.rc site found at 3751
    Illegal BsaI.rc site found at 3763
    Illegal BsaI.rc site found at 4630
    Illegal SapI.rc site found at 3435
    Illegal SapI.rc site found at 4017
    Illegal SapI.rc site found at 4032


Design Notes

None


Source

CLB2 promoter is from the genome of Saccharomyces cerevisiae BY4741.

Cas9 is from the pRH003 plasmid.


References