Difference between revisions of "Part:BBa K3468086:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro, and Ser58 is at the i+1 position of β-turn. The introduction of proline mutations can theoretically improve thermal stability. | |
Latest revision as of 11:14, 24 October 2020
PETase S58P
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 75
Design Notes
Reading the literature found that the i+1 position of β-turn has an obvious residue preference for pro, and Ser58 is at the i+1 position of β-turn. The introduction of proline mutations can theoretically improve thermal stability.
Source
Ideonalla sakaiensis