Difference between revisions of "Part:BBa K3431015"

 
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This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.  
 
This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.  
  
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===Experiment result===
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<img src="https://static.igem.org/mediawiki/parts/f/f8/T--CSMU_Taiwan--Fig._4_%28EXP_2%29.png" style="width:100%">
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:49, 24 October 2020


T7 Promoter + RBS + Thermotoga maritima Invertase + T7 Terminator

This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.

Experiment result


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1127
    Illegal BamHI site found at 1257
    Illegal XhoI site found at 1328
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 928
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 459