Difference between revisions of "Part:BBa K3431015"
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This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit. | This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit. | ||
+ | ===Experiment result=== | ||
+ | <html> | ||
+ | <div style="width=100%; display:flex; align-items: center; justify-content: center"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/f/f8/T--CSMU_Taiwan--Fig._4_%28EXP_2%29.png" style="width:100%"> | ||
+ | </div> | ||
+ | <br> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 07:49, 24 October 2020
T7 Promoter + RBS + Thermotoga maritima Invertase + T7 Terminator
This is a protein generator that expresses a hydrolytic protein - Beta-Fructosidase (Invertase, BBa_K3431000). We added T7 Promoter (BBa_I719005) and RBS (BBa_B0034) upstream as well as T7 terminator (BBa_K731721) downstream of Beta-Fructosidase to insure its ability of transcription and translation in PURExpress in vitro Protein Synthesis Kit.
Experiment result
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1356
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1127
Illegal BamHI site found at 1257
Illegal XhoI site found at 1328 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 928
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 459