Difference between revisions of "Part:BBa I766557"
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Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)
 
Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)
  
−
===Usage and Biology===
+
==Usage and Biology==
 +
===Estonia TUIT Contribution===
 
The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 ('''Figure 1'''). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.  
 
The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 ('''Figure 1'''). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.  
 
[[File:1(2).jpg|thumb|center|500px|Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009).
 
[[File:1(2).jpg|thumb|center|500px|Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009).

Revision as of 04:47, 24 October 2020


pSte5 (Weak) Promoter

Low expression, constitutive promoter in yeast
Induced by α-factor-pheromone (Printen & Sprague, 1994)
Ste5 scaffold protein; ensures assembly of the protein kinases of the pheromone-activated MAPK cascade into an active complex during mating (Printen & Sprague, 1994)

Usage and Biology

Estonia TUIT Contribution

The comparative study on the abundance of the Ste5 scaffold protein depending on the promoter was conducted by Chapman & Asthagiri in 2009 (Figure 1). As can be seen from Figure 1, STE5 promoter is a very weak promoter, which ensures a lower expression level in comparison even with CYC promoter.

Figure 1. The relative abundance of STE5-MYC-tagged protein expressed from inducible STE5 (induced by α-factor) and constitutive CYC, ADH, TEF, and GPD promoters. The error bars represent SEM (n=3) (Chapman & Asthagiri, 2009).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
  • 1000
    COMPATIBLE WITH RFC[1000]

References
Chapman, S. A., & Asthagiri, A. R. (2009). Quantitative effect of scaffold abundance on signal propagation. Molecular Systems Biology, 5(1), 313. https://doi.org/10.1038/msb.2009.73
Printen, J. A., & Sprague, G. F. (1994). Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade. Genetics, 138(3), 609–619.