Difference between revisions of "Part:BBa K3580102"

Revision as of 04:19, 24 October 2020

Ptrc-trGPPS-SS(Sabinene synthase)

It is a part composed of trGPPS and sabinene synthase whose expression is regulated by Ptrc, and LacI to enable IPTG inductionof expression of those enzymes.This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in a 2015 paper by Srividya, Narayanan et al. changed the limonene synthase of Ptrc-trGPPS-LS to sabinene synthase.Sabinenecan be synthesized by combining this part with an upstream pathway that can supply the products of the mevalonate pathway (IPP and DMAPP).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1980
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1980
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2366
Illegal XhoI site found at 4022
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1980
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1980
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3580102 short</partinfo>
-iGEM waseda 2020  long description test
+It is a part composed of trGPPS and sabinene synthase whose expression is regulated by Ptrc, and LacI to enable IPTG inductionof expression of those enzymes.This part was designed based on the Ptrc-trGPPS-LS(BBa_K3580101)used in the 2013 paper by Alonso-Gutierrez, Jorge et al. The introduction of the one-amino acid mutation reported in a 2015 paper by Srividya, Narayanan et al. changed the limonene synthase of Ptrc-trGPPS-LS to sabinene synthase.Sabinenecan be synthesized by combining this part with an upstream pathway that can supply the products of the mevalonate pathway (IPP and DMAPP).
 <!-- Add more about the biology of this part here