Difference between revisions of "Part:BBa K3697000"

Revision as of 00:16, 24 October 2020

mCherry BSU

This is the coding sequence for producing a codon-optimized mCherry in B. subtilis. mCherry is a derivative of RFP. mCherry_BSU was created by codon-optimizing an E. coli mCherry for expression B. subtilis. When paired with a strong RBS and constitutive promotor (BBa_K3697010), mCherry_BSU can be quantified using a plate reader, and transformed B. subtilis colonies can be moderately red to the naked eye.

mCherry_BSU has an excitation peak at 585 nm and a peak emission at 615 nm.

Fluorescence levels in B. subtilis and E. coli can be quantified using a fluorescent plate reader. Under high enough expression from a strong constitutive promotor such as pVeg (BBa_K143012), red colonies may be visible to the naked eye. E. coli expressing mCherry_BSU are red under natural light.

This protein can be used as a reporter in B. subtilis due to it's high visibility.

This part does not have a degradation tag.

Sequence and Features