Difference between revisions of "Part:BBa K3360002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies. | + | Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons |
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===Source=== | ===Source=== | ||
| − | + | Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons | |
===References=== | ===References=== | ||
| + | Zhang Hui, Qiu Riyong, Liu Mingjie, et al. Cloning and overexpression of extremely heat-resistant β-glucosidase gene and its application in the industrial production of natural blue pigment[J].Jiangsu Agricultural Sciences,2013,41(7 ):19-22. | ||
Latest revision as of 22:04, 23 October 2020
Thermostable beta-glucosidase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1577
Illegal AgeI site found at 927 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons
Source
Encoding a β-glucosidase derived from Thermotoga maritima. Its heat resistance has been greatly improved, and the optimum temperature is 90°C.The N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.The entire sequence has been optimized by E.coli codons
References
Zhang Hui, Qiu Riyong, Liu Mingjie, et al. Cloning and overexpression of extremely heat-resistant β-glucosidase gene and its application in the industrial production of natural blue pigment[J].Jiangsu Agricultural Sciences,2013,41(7 ):19-22.