Difference between revisions of "Part:BBa K3346000:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Site.
+
We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite.
  
  

Latest revision as of 21:26, 23 October 2020


Siltuximab Heavy Variable Chain for IgG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite.


Source

This nucleotide sequence was converted from amino acid sequence and optimized for expression in E. coli using available online software.

Organism: chimeric (mouse and human)

References