Difference between revisions of "Part:BBa K3346000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. | + | We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite. |
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This nucleotide sequence was converted from amino acid sequence and optimized for expression in E. coli using available online software. | This nucleotide sequence was converted from amino acid sequence and optimized for expression in E. coli using available online software. | ||
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| + | Organism: chimeric (mouse and human) | ||
===References=== | ===References=== | ||
Latest revision as of 21:26, 23 October 2020
Siltuximab Heavy Variable Chain for IgG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used alternative codons in order to remove illegal restriction sites and optimize the sequence for all available BioBrick standards. We also performed codon optimization for an E. coli chassis using Reverse Translate Software available on The Sequence Manipulation Suite.
Source
This nucleotide sequence was converted from amino acid sequence and optimized for expression in E. coli using available online software.
Organism: chimeric (mouse and human)