Difference between revisions of "Part:BBa K3380500:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase. | + | All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase. |
| + | The method was similar to the one used by Millacura ''et al.'' 2019. (for more details check the experience page) | ||
===Source=== | ===Source=== | ||
| − | + | The parts were acquired as ''de novo'' synthesized sequences from Sigma-Aldrich. | |
| − | + | ||
===References=== | ===References=== | ||
| + | Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230. | ||
Latest revision as of 18:23, 23 October 2020
iSpinach fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 25
Design Notes
All the sequences for the individual parts were acquired. The individual part were designed with 4bp scars to allow swapping of the parts to test different constructs. The individual parts were phosphorylated, annealed and ligated together using the T4 ligase. The method was similar to the one used by Millacura et al. 2019. (for more details check the experience page)
Source
The parts were acquired as de novo synthesized sequences from Sigma-Aldrich.
References
Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230.