Difference between revisions of "Part:BBa K2520045"
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PLA2 enzymes are commonly found in mammalian tissues as well as insect venom. The venom is largely composed of melittin, which is a stimulant of PLA2. Due to the increased presence and activity of PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site (1). | PLA2 enzymes are commonly found in mammalian tissues as well as insect venom. The venom is largely composed of melittin, which is a stimulant of PLA2. Due to the increased presence and activity of PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site (1). | ||
| − | [[File:pla.png| | + | [[File:pla.png|200px|thumb|center|Figure 1: Phospholipid with phospholipase cleavage sites (in orange)]] |
===Epitopes=== | ===Epitopes=== | ||
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[[File:pla_epitopes.png|500px|thumb|center|Figure 2: 62 sequentially overlapping dodecapeptides (12 aa) representing the full length of the PLA molecule. The highest immunogenic response was of the 81-92 and 113-124 epitopes (2).]] | [[File:pla_epitopes.png|500px|thumb|center|Figure 2: 62 sequentially overlapping dodecapeptides (12 aa) representing the full length of the PLA molecule. The highest immunogenic response was of the 81-92 and 113-124 epitopes (2).]] | ||
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| + | ==MIT_MAHE 2020== | ||
| + | '''Summary''' | ||
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| + | Bee venom PLA epitope 1 has been used in immunotherapy being highly effective. But allergic side effects have been known to occur during the treatment. Phospholipase A2 (PLA) is the major allergen of honey bee venom (BV). | ||
Latest revision as of 18:03, 23 October 2020
Bee venom PLA epitope 1
Introduction
The major Bee Venom (BV) allergen is the phospholipase A2 (PLA). Phospholipases A2 (PLA2s) are enzymes that release fatty acids from the second carbon group of glycerol. PLA2 enzymes are commonly found in mammalian tissues as well as insect venom. The venom is largely composed of melittin, which is a stimulant of PLA2. Due to the increased presence and activity of PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site (1).
Epitopes
PLA 1 (our counting), is the sequence of one of the most immunogenic epitopes of PLA protein, amino acids 113-124.
MIT_MAHE 2020
Summary
Bee venom PLA epitope 1 has been used in immunotherapy being highly effective. But allergic side effects have been known to occur during the treatment. Phospholipase A2 (PLA) is the major allergen of honey bee venom (BV).
References
(1) Müller, Ulrich, et al. "Successful immunotherapy with T-cell epitope peptides of bee venom phospholipase A2 induces specific T-cell anergy in patients allergic to bee venom." Journal of allergy and clinical immunology 101.6 (1998): 747-754.
(2) Carballido, J. M., et al. "T cell epitope specificity in human allergic and nonallergic subjects to bee venom phospholipase A2." The Journal of Immunology 150.8 (1993): 3582-3591.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]