Difference between revisions of "Part:BBa K3482011"
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<partinfo>BBa_K3482011 short</partinfo>
 
<partinfo>BBa_K3482011 short</partinfo>
  
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The Ptac promoter sequence here is preceeded by a coding region for lacI; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state.
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The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.
  
 
We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
 
We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.

Revision as of 16:32, 23 October 2020


pTac promoter

The pTac promoter sequence here is preceded by a binding region (operator) for the repressor LacI; the binding of LacI prevents transcription. Transcription from pTac is enabled by the addition of IPTG, which binds to LacI and releases it from its operator.

We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]