Difference between revisions of "Part:BBa K3505009"

Revision as of 15:24, 23 October 2020

pDGB1 alpha 2 (α2) for Bacterial GoldenBraid Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Kanamycin and ori pBBR1 for multiple protein expression capacities.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3224
Illegal XbaI site found at 3251
Illegal PstI site found at 3263
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3224
Illegal PstI site found at 3263
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3224
Illegal BamHI site found at 3245
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3224
Illegal XbaI site found at 3251
Illegal PstI site found at 3263
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3224
Illegal XbaI site found at 3251
Illegal PstI site found at 3263
Illegal NgoMIV site found at 1291
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3463
Illegal BsaI.rc site found at 2881

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3505009 short</partinfo>
+Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening between BsaI and BsmBI sites.Resistance in Kanamycin and ori pBBR1 for multiple protein expression capacities.
-LONG
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+===Sequence and Features===
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3505009 SequenceAndFeatures</partinfo>
-<!-- Uncomment this to enable Functional Parameter display
-===Functional Parameters===
 <partinfo>BBa_K3505009 parameters</partinfo>
-<!-- -->