Difference between revisions of "Part:BBa K3402002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | We used green fluorescence protein <i>yeGFP</i>([https://parts.igem.org/Part:BBa_K3402000# | + | We used green fluorescence protein <i>yeGFP</i> ([https://parts.igem.org/Part:BBa_K3402000#_BBa_K3402000]) to characterize the strength of this promoter in <i>S. bombicola</i>. |
[[Image:Expression of Ppgi by yeGFP.png|700px|thumb|center|Expression of P<i>pgi</i> by <i>yeGFP</i>]] | [[Image:Expression of Ppgi by yeGFP.png|700px|thumb|center|Expression of P<i>pgi</i> by <i>yeGFP</i>]] |
Revision as of 13:47, 23 October 2020
Ppgi
Ppgi is the promoter of glucose-6-phosphate isomerase from Starmerella bombicola.
Usage and Biology
We used green fluorescence protein yeGFP ([1]) to characterize the strength of this promoter in S. bombicola.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 259
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 259
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 259
Illegal BglII site found at 837 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 259
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 259
- 1000COMPATIBLE WITH RFC[1000]