Difference between revisions of "Part:BBa K3468019:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NONE
+
By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. N275F was designed by searching for suitable sites around F284 for mutation.
  
  

Revision as of 13:15, 23 October 2020


PETase N275F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. N275F was designed by searching for suitable sites around F284 for mutation.


Source

Ideonalla sakaiensis

References