Difference between revisions of "Part:BBa K079041"

Revision as of 12:27, 29 October 2008

Lambda OR1 - operator library member

This parts have been extracted from the Lambda Operator Library.

Right Regulatory Elements position in natural cI genetic switch

Lambda Fage Genetic switch is a complex and deeply characterized system. A Review on the natural systems behavior

Test System used to assay Biological Activity

ΔG of cI Binding to it's different operator sites and resulting Biological repression strength expressed as reduction in LacZ Activity

K079041: Lambda OR1 is the most affine wild type operator sequence in the Lambda system. In the natural switch overlaps with Cro Promoter PR.

K079042: Lambda OR2 is located upstream of OR1 partially overlapping the PR promoter. cI binding affinity for the operator sequence is significantly lower than OR1 (Higher Free Energy)

K079043: Lambda OR3 recruits cI with interaction energy comparable to OR2. The different biological role in the switch systems is related to positional effects being OR3 farther from PR sequence and overlapping cI promoter P.

The resuming table clearly shows as to determine the actual repression strength both binding affinity and [http://2008.igem.org/Team:Bologna/Modeling Positional Effects] are key elements. For Example OR3 has the strongest repression despite the lower binding affinity and has very different repression efficiency compared to the similarly binding OR2.

Sequence and Features