Difference between revisions of "Part:BBa K3510002"

Revision as of 12:12, 23 October 2020

Mn-Promoter-Mn-Riboswitch-FAST-Chromoprotein-Terminator

Important: This construct does not include a RBS upstream of the FAST2 sequence. When building on our design please make sure you add it in order to allow for translation

This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: the manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).

Usage: In our project we coupled this manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This allows in field testing without the necessity of a laboratory providing a fluorescence plate reader. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure. Additionally, this construct was designed as a control for the effect of phytochelatin on the sensing mechanism in the construct BBa_K3510003. Unlike the phytochelatin the chromoprotein should not interfere with manganese.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 319

@@ Line 1: / Line 1: @@
 <partinfo>BBa_K3510002 short</partinfo>
-This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: The manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation.  Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).
+<b><font color= red>Important: This construct does not include a RBS upstream of the FAST2 sequence. When building on our design please make sure you add it in order to allow for translation</font color= red></b>
+This composite part is a manganese inducible expression system of a blue chromoprotein (BBa_K864401) additionally tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: the manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).
 <b>Usage:</b>
-In our project we coupled the manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This should allow in field testing without the necessity of a laboratory providing a equipment. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure.
+In our project we coupled this manganese sensing device with a chromoprotein to have an easy-to-use biosensor that gives a qualitative signal by color. This allows in field testing without the necessity of a laboratory providing a fluorescence plate reader. Therefore, this construct could be really beneficial to roughly examine water quality in areas with poor infrastructure.
 Additionally, this construct was designed as a control for the effect of phytochelatin on the sensing mechanism in the construct BBa_K3510003. Unlike the phytochelatin the  chromoprotein should not interfere with manganese.