Difference between revisions of "Part:BBa K079041"

Revision as of 11:24, 29 October 2008

Lambda OR1 - operator library member

This parts have been extracted from the Lambda Operator Library.

Regulatory Elements position in natural cI genetic switch

K079041: Lambda OR1 is the most affine wild type operator sequence in the Lambda system. In the natural switch overlaps with Cro Promoter PR.

K079042: Lambda OR2 is located upstream of OR1 partially overlapping the PR promoter. cI binding affinity for the operator sequence is significantly lower than OR1 (Higher Free Energy)

K079043: Lambda OR3 recruits cI with interaction energy comparable to OR2. The different biological role in the switch systems is related to positional effects being farther from PR sequence and overlapping cI promoter P.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K079041 short</partinfo>
-..
+This parts have been extracted from the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079047 Lambda Operator Library].
+[[Image:cIswitch.jpg |left|350px|thumbnail|Regulatory Elements position in natural cI genetic switch]]
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079041 K079041]: Lambda OR1 is the most affine wild type operator sequence in the Lambda system. In the natural switch overlaps with Cro Promoter PR.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079042 K079042]: Lambda OR2 is located upstream of OR1 partially overlapping the PR promoter. cI binding affinity for the operator sequence is '''significantly lower than OR1''' (Higher Free Energy)
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079043 K079043]: Lambda OR3 recruits cI with interaction energy comparable to OR2. The different biological role in the switch systems is related to positional effects being farther from PR sequence and overlapping cI promoter P.
 <!-- Add more about the biology of this part here