Difference between revisions of "Part:BBa K3596010"
 
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<h6 style="text-align:center">Figure 1:  <strong>A</strong> Construction of BIOT gene circuit</h6>   
 
<h6 style="text-align:center">Figure 1:  <strong>A</strong> Construction of BIOT gene circuit</h6>   
 
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===Characterization===
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<p>In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system. This part codes for the  
 
<p>In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system. This part codes for the  
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  mutated RBS of hofB gene in BIOT system, with the strength enhanced by 10 folds (figure 2).
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  mutated RBS of hofB gene in BIOT system, with the strength enhanced by 10 folds calculated by RBS calculator.
 
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<img src="https://2020.igem.org/wiki/images/1/13/T--GreatBay_SZ--RBS.png" alt="" width="700">
 
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<h6 style="text-align:center">Figure 2:  Different pilin productivity of engineered BIOT system with different RBS. </h6> 
 
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Latest revision as of 11:20, 23 October 2020

hofB RBS mut1

BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.

Figure 1: A Construction of BIOT gene circuit

In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system. This part codes for the mutated RBS of hofB gene in BIOT system, with the strength enhanced by 10 folds calculated by RBS calculator.

References

[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]