Difference between revisions of "Part:BBa K3423000"
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| − | This Aca1 protein is the inhibitor of Anti-CRISPR (acr) promoter, it was identified in 2019 (Stanley et al., 2019). | + | This anti-CRISPR-associated 1 (Aca1) protein is the inhibitor of Anti-CRISPR (acr) promoter, it was identified in 2019 (Stanley et al., 2019). |
| − | + | Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially. | |
| + | We characterised the efficiency of this part of inhibiting a downstream promoter, it successfully worked in our composite part [https://parts.igem.org/Part:BBa_K3423005 BBa_K3423005].The inhibition of Aca1 is measured through green fluorescent protein: https://static.igem.org/mediawiki/parts/3/33/SUSTech_ShenzhenGFP.png | ||
| + | |||
| + | ==Reference== | ||
Stanley, S. Y., Borges, A. L., Chen, K.-H., Swaney, D. L., Krogan, N. J., Bondy-Denomy, J., & Davidson, A. R. (2019). Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription. Cell, 178(6), 1452-1464.e1413. doi:10.1016/j.cell.2019.07.046 | Stanley, S. Y., Borges, A. L., Chen, K.-H., Swaney, D. L., Krogan, N. J., Bondy-Denomy, J., & Davidson, A. R. (2019). Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription. Cell, 178(6), 1452-1464.e1413. doi:10.1016/j.cell.2019.07.046 | ||
| + | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 11:11, 23 October 2020
Anti-CRISPR (acr) promoter inhibitor
This anti-CRISPR-associated 1 (Aca1) protein is the inhibitor of Anti-CRISPR (acr) promoter, it was identified in 2019 (Stanley et al., 2019).
Protein anti-CRISPR-associated 1 (Aca1) is expressed by T7 promoter, Aca1 will subsequently inhibit the anti-CRISPR promoter, so the gene expression downstream anti-CRISPR will be inhibited until Navitoclax (a compound that can inhibit Aca1 found by SUSTech_Shenzhen) is added artificially. Without the inhibition from Aca1, the expression of genes downstream anti-CRISPR promoter will increase significantly. Therefore, protein expression can be regulated artificially.
We characterised the efficiency of this part of inhibiting a downstream promoter, it successfully worked in our composite part BBa_K3423005.The inhibition of Aca1 is measured through green fluorescent protein: 
Reference
Stanley, S. Y., Borges, A. L., Chen, K.-H., Swaney, D. L., Krogan, N. J., Bondy-Denomy, J., & Davidson, A. R. (2019). Anti-CRISPR-Associated Proteins Are Crucial Repressors of Anti-CRISPR Transcription. Cell, 178(6), 1452-1464.e1413. doi:10.1016/j.cell.2019.07.046
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]