Difference between revisions of "Part:BBa K3505008"

Revision as of 09:37, 23 October 2020

pDGB1 alpha 1R (α1R) for Bacterial GoldenBraid.

Level a vector for Golden Braid Cloning. Having cargo LacZa for blue white screening.

Fig.1: a1R plasmid Features and Restiction Sites.

Fig.2: a1R Map.

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3118
Illegal XbaI site found at 3091
Illegal PstI site found at 3079
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3118
Illegal PstI site found at 3079
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3118
Illegal BamHI site found at 11
Illegal BamHI site found at 2858
Illegal BamHI site found at 3097
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3118
Illegal XbaI site found at 3091
Illegal PstI site found at 3079
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3118
Illegal XbaI site found at 3091
Illegal PstI site found at 3079
Illegal NgoMIV site found at 1290
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3461
Illegal BsaI.rc site found at 2879

@@ Line 9: / Line 9: @@
 [[File:T--Thessaly--a1r.png|500px|thumb|left|<i><b>Fig.1:</b> a1R plasmid Features and Restiction Sites.</i>]]
-[[File:T--Thessaly--a1r_map.png|500px|thumb|left|<i><b>Fig.2:</b> a1R Map.</i>]]
+[[File:T--Thessaly--a1r_map.png|500px|thumb|right|<i><b>Fig.2:</b> a1R Map.</i>]]
 ===Sequence and Features===
 <partinfo>BBa_K3505008 SequenceAndFeatures</partinfo>