Difference between revisions of "Part:BBa K3573001:Design"

 
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===Design Notes===
 
===Design Notes===
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CPP was attached to the protein for entry into the cell
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Last year, Korea_HS team has modelled the structure of CPP-scFv(RAS) using the program Modeller. Modeller used searched the database and used similar structures (PDB ID: 5B3N, 6NJL, 5GS3, 6G8R and 3UYP) to build a homology model. Based on the results of the homology modelling, we have decided to synthesize the gene for CPP-scFv(RAS) for protein production and binding assays.
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CPP (BBa_K3090000) was attached to scFv(RAS)(BBa_3573000) for entry into the cell.
  
 
===Source===
 
===Source===

Latest revision as of 04:24, 23 October 2020


Single chain variable fragment with CPP recognizing RAS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Last year, Korea_HS team has modelled the structure of CPP-scFv(RAS) using the program Modeller. Modeller used searched the database and used similar structures (PDB ID: 5B3N, 6NJL, 5GS3, 6G8R and 3UYP) to build a homology model. Based on the results of the homology modelling, we have decided to synthesize the gene for CPP-scFv(RAS) for protein production and binding assays.

CPP (BBa_K3090000) was attached to scFv(RAS)(BBa_3573000) for entry into the cell.

Source

DNA was synthesized

References