Difference between revisions of "Part:BBa K3573002:Experience"
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===Applications of BBa_K3573002=== | ===Applications of BBa_K3573002=== | ||
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| + | Korea_HS team have performed binding assays using size-exclusion chromatography. We ran three separate runs of 3 superdex75 10/300GL column with buffer (20mM Tris-HCl pH 7.0+250mM NaCl) | ||
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| + | 1. scFv(RAS) only | ||
| + | 2. HRAS only | ||
| + | 3. scFv(RAS)+HRAS | ||
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| + | The result showed that HRAS's elution peak shifted to higher molecular weight position in the presence of scFv(RAS) compared to HRAS only, indicating their binding. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 04:20, 23 October 2020
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Applications of BBa_K3573002
Korea_HS team have performed binding assays using size-exclusion chromatography. We ran three separate runs of 3 superdex75 10/300GL column with buffer (20mM Tris-HCl pH 7.0+250mM NaCl)
1. scFv(RAS) only 2. HRAS only 3. scFv(RAS)+HRAS
The result showed that HRAS's elution peak shifted to higher molecular weight position in the presence of scFv(RAS) compared to HRAS only, indicating their binding.
User Reviews
UNIQc110212774f0472a-partinfo-00000000-QINU UNIQc110212774f0472a-partinfo-00000001-QINU