Difference between revisions of "Part:BBa K3503001:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
The milk protein α-s1-casein from Bos taurus with his tag
+
By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE).
 
+
 
+
  
 
===Source===
 
===Source===
 
+
The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus;
The milk protein α-s1-casein from Bos taurus with his tag
+
  
 
===References===
 
===References===

Latest revision as of 03:31, 23 October 2020


Alpha-s1-casein-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 643
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE).

Source

The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus;

References