Difference between revisions of "Part:BBa K3699001"
Line 3: Line 3:
 
<html>
 
<html>
 
<figure><img src="https://static.igem.org/mediawiki/parts/c/c5/T--BUCT--team-logo-small.jpeg"/></figure>
 
<figure><img src="https://static.igem.org/mediawiki/parts/c/c5/T--BUCT--team-logo-small.jpeg"/></figure>
−
 
 
</html>
 
</html>
 +
 
=='''Introduction'''==
 
=='''Introduction'''==
 
<html>
 
<html>
Line 16: Line 16:
  
 
<figure><img src="https://static.igem.org/mediawiki/2014/0/05/Peking2014jyj_2.png"/><figcaption><b>Figure 1. First step of biodegradation of MC-LR.</b> MlrA mediates breaking peptide bond between Adda and Arg, which leads to significant decrease of toxicity.<sup>[1]</sup></figcaption></figure>
 
<figure><img src="https://static.igem.org/mediawiki/2014/0/05/Peking2014jyj_2.png"/><figcaption><b>Figure 1. First step of biodegradation of MC-LR.</b> MlrA mediates breaking peptide bond between Adda and Arg, which leads to significant decrease of toxicity.<sup>[1]</sup></figcaption></figure>
−
 
 
</html>
 
</html>
 +
 +
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
Line 42: Line 43:
 
<p>[1] Li J , Li R , Li J . Current research scenario for microcystins biodegradation - A review on fundamental knowledge, application prospects and challenges[J]. <i>ence of the Total Environment</i>, 2017, 595(OCT.1):615.</p>
 
<p>[1] Li J , Li R , Li J . Current research scenario for microcystins biodegradation - A review on fundamental knowledge, application prospects and challenges[J]. <i>ence of the Total Environment</i>, 2017, 595(OCT.1):615.</p>
 
<p>[2] Gehringer M M , Milne P , Lucietto F , et al. Comparison of the structure of key variants of microcystin to vasopressin[J]. <i>Environmental Toxicology & Pharmacology</i>, 2005, 19(2):297-303.</p>
 
<p>[2] Gehringer M M , Milne P , Lucietto F , et al. Comparison of the structure of key variants of microcystin to vasopressin[J]. <i>Environmental Toxicology & Pharmacology</i>, 2005, 19(2):297-303.</p>
 +
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K3699001 SequenceAndFeatures</partinfo>
 +
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K1378001 parameters</partinfo>
+
<partinfo>BBa_K3699001 parameters</partinfo>
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K1378001 SequenceAndFeatures</partinfo>
 

Revision as of 01:31, 23 October 2020

MlrA from Novosphingobium sp. THN1

Introduction

This part is microcystin enzyme (MlrA) from Novosphingobium sp. THN1.

MlrA is an enzyme that degrades cyanobacterial toxins, especially microcystin-LR (MC-LR).

By comparing the ability of Sphingomonas sp. ACM-3962, Novosphingobium sp. THN1 and other bacteria’s data, we found Novosphingobium sp. THN1 shows a stronger activity. So we characterize it.

To promote enzyme expression, we replaced the commonly used strong promoter J23110 with the stronger J23119 in its family. This part successfully expressed MlrA enzyme and showed activity on degrading MC-LR.

Figure 1. First step of biodegradation of MC-LR. MlrA mediates breaking peptide bond between Adda and Arg, which leads to significant decrease of toxicity.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]