Difference between revisions of "Part:BBa K3338005:Design"

 
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===Design Notes===
 
===Design Notes===
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The original sequence of the IL6P was modified so that it is compatible with the RFC10 assembly standard. Therefore, a point mutation in a XbaI site was introduced. There is no predicted clash with a NF-κB or an AP1 transcription factor binding site.
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The original sequence of the human IL-6 P was modified so that it is compatible with the RFC10 assembly standard. Therefore, a point mutation in a XbaI site was introduced. There is no predicted clash with a NF-κB or an AP1 transcription factor binding site.
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===Source===
 
===Source===

Latest revision as of 21:03, 22 October 2020


Interleukin-6 Promoter mutagenized (IL-6 Pmut)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Design Notes

The original sequence of the human IL-6 P was modified so that it is compatible with the RFC10 assembly standard. Therefore, a point mutation in a XbaI site was introduced. There is no predicted clash with a NF-κB or an AP1 transcription factor binding site.

Source

The sequence was synthesized by IDT.

References