Difference between revisions of "Part:BBa K117003:Design"

(Design Notes)
 
(6 intermediate revisions by 2 users not shown)
Line 10: Line 10:
 
These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.
 
These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.
  
 +
The T7ptag was derived from the original AND gate obtained from Voigt lab.
  
 +
Plasmid pBACr-Mgr940 containing t7ptag derived from plasmid pBAC874t was generously donated by voigt lab. T7ptag is extracted from plasmid pBACr-Mgr940 using PCR with oligo sequences of 5'-CCTAGGAATTAACCGTGACCATGA-3' & 3'-CTGAAGCGCAAGCGTATT-5' with biobricks prefix and suffix respectively.
  
 
===Source===
 
===Source===
Line 17: Line 19:
  
 
===References===
 
===References===
Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)
+
Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)

Latest revision as of 05:44, 29 October 2008

T7ptag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).

These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.

The T7ptag was derived from the original AND gate obtained from Voigt lab.

Plasmid pBACr-Mgr940 containing t7ptag derived from plasmid pBAC874t was generously donated by voigt lab. T7ptag is extracted from plasmid pBACr-Mgr940 using PCR with oligo sequences of 5'-CCTAGGAATTAACCGTGACCATGA-3' & 3'-CTGAAGCGCAAGCGTATT-5' with biobricks prefix and suffix respectively.

Source

[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]

References

Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)