Difference between revisions of "Part:BBa K117003:Design"
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The T7ptag was derived from the original AND gate obtained from Voigt lab. | The T7ptag was derived from the original AND gate obtained from Voigt lab. | ||
| − | + | Plasmid pBACr-Mgr940 containing t7ptag derived from plasmid pBAC874t was generously donated by voigt lab. T7ptag is extracted from plasmid pBACr-Mgr940 using PCR with oligo sequences of 5'-CCTAGGAATTAACCGTGACCATGA-3' & 3'-CTGAAGCGCAAGCGTATT-5' | |
===Source=== | ===Source=== | ||
Revision as of 05:37, 29 October 2008
T7ptag
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7ptag is responsible for the transcription of T7 RNA polymerase. The polymerase gene has been modified to contain two amber stop codons (T7ptag).
These stop codons are translated as serine when supD is also transcribed. Polymerase is expressed only when both SupD and T7ptag mRNA are present.
The T7ptag was derived from the original AND gate obtained from Voigt lab.
Plasmid pBACr-Mgr940 containing t7ptag derived from plasmid pBAC874t was generously donated by voigt lab. T7ptag is extracted from plasmid pBACr-Mgr940 using PCR with oligo sequences of 5'-CCTAGGAATTAACCGTGACCATGA-3' & 3'-CTGAAGCGCAAGCGTATT-5'
Source
[http://www.voigtlab.ucsf.edu/index.html http://www.voigtlab.ucsf.edu/index.html]
References
Anderson JC, Voigt CA, Arkin AP (2007) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1964800 Environmental signal integration by a modular AND gate], Molecular Systems Biology, 3 (133)