Difference between revisions of "Part:BBa K3570001"
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<h2>Design</h2>
 
<h2>Design</h2>
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[[File:BBa K3570001-1.png|600px|thumb|center|Fig. 1: Brazzein-CrGES-pUC19. The integrative locus used is yprcΔ15. The selective locus used is LEU2. The gene coding for geraniol synthase (CrGES) comes from <I>C. roseus</I>. The gene coding for brazzein comes from an artificial sequence (LG263246.1). CYC1 and PGK terminators are used. The bidirectional promoter Gal 1/10 is used. ]]
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<h2>Experiments</h2>
 
<h2>Experiments</h2>

Revision as of 19:31, 22 October 2020


Sweet rose taste induction system in S. cerevisiae

Introduction

Design

Fig. 1: Brazzein-CrGES-pUC19. The integrative locus used is yprcΔ15. The selective locus used is LEU2. The gene coding for geraniol synthase (CrGES) comes from C. roseus. The gene coding for brazzein comes from an artificial sequence (LG263246.1). CYC1 and PGK terminators are used. The bidirectional promoter Gal 1/10 is used.


Experiments

References


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4894
    Illegal BamHI site found at 3752
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
    Illegal AgeI site found at 1551
    Illegal AgeI site found at 4507
  • 1000
    COMPATIBLE WITH RFC[1000]