Difference between revisions of "Part:BBa K3570012"

Latest revision as of 14:42, 22 October 2020

Geraniol synthase from Catharanthus roseus

Usage

Catharanthus roseus produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes the transformation of geranyl diphosphate (GPP) into geraniol in the monoterpenoid branch of the MIA pathway.

The sequence of the geraniol synthase from Catharanthus roseus (CrGES) has been taken from Genbank (JN882024.1), adapted for the usage in S. cerevisiae and then truncated to the 43 amino acid in order to increase its activity (Jiang et al., 2017).

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

Related parts on regisrty

There is another part in registry which corresponds to geraniol synthase (BBa_K2753003). The two main differences with ours are that the sequence originates from Ocimum basilica and that the codons are adapted for the usage in E.coli.

References

GenBank ID: JN882024.1

Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3570012 short</partinfo>
+<h2>Usage</h2>
 <p style="text-indent: 40px">
-Catharanthus roseus produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes a key step in the generation of geraniol from geranyl diphosphate (GPP) in the monoterpenoid branch of the MIA pathway.</p>
+<i>Catharanthus roseus</i> produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes the transformation of geranyl diphosphate (GPP) into geraniol in the monoterpenoid branch of the MIA pathway.</p>
 <p style="text-indent: 40px">
-The sequence of the geraniol synthase from Catharanthus roseus (CrGES) has been taken from Genbank (JN882024.1) then truncated to the 43 amino acid in order to increase its activity (Jiang et al.).</p>
+The sequence of the geraniol synthase from <i>Catharanthus roseus</i> (CrGES) has been taken from Genbank (JN882024.1), adapted for the usage in <i>S. cerevisiae</i> and then truncated to the 43 amino acid in order to increase its activity (Jiang <i>et al</i>., 2017).</p>
-References:
+<h2>Experiments</h2>
-- GenBank ID: JN882024.1
+Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
-- Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005
+<h2>Related parts on regisrty</h2>
+<p style="text-indent: 40px">
+There is another part in registry which corresponds to geraniol synthase ([https://parts.igem.org/Part:BBa_K2753003 BBa_K2753003]). The two main differences with ours are that the sequence originates from <i>Ocimum basilica</i> and that the codons are adapted for the usage in <i>E.coli</i>.</p>
+<h2>References</h2>
+* GenBank ID: JN882024.1
+* Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3570012 SequenceAndFeatures</partinfo>