Difference between revisions of "Part:BBa K3570015"

 
 
(4 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3570015 short</partinfo>
 
<partinfo>BBa_K3570015 short</partinfo>
 +
<p style="text-indent: 40px">
 +
YPRCdelta15 downstream homologous arm part shall be used together with YPRCdelta15 upstream homology arm part ([https://parts.igem.org/Part:BBa_K3570014 BBa_K3570014]) to target a functional yeast integration locus. When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the <em>S. cerevisiae</em>'s genome. It will do homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI. </p>
 +
 +
<h2>Experiments</h2>
 +
Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.
 +
<h2>References</h2>
 +
*Bai Flagfeldt, D., Siewers, V., Huang, L., & Nielsen, J. (2009). Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae. Yeast, 26(10), 545–551. https://doi.org/10.1002/yea.1705
 +
*<i>S. cerevisiae</i> genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1
 +
  
−
YPRCdelta15 downstream homologous arm used together with YPRCdelta15 upstream (BBa_K3570014). When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:36, 22 October 2020


YPRCdelta15 downstream homologous sequnce

YPRCdelta15 downstream homologous arm part shall be used together with YPRCdelta15 upstream homology arm part (BBa_K3570014) to target a functional yeast integration locus. When YPRCdelta15 upstream put to 5' of the biobrick together with YPRCdelta15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within a region of type 1 long terminal repeat (Ty1 LTR) in chromosome XVI.

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References

  • Bai Flagfeldt, D., Siewers, V., Huang, L., & Nielsen, J. (2009). Characterization of chromosomal integration sites for heterologous gene expression inSaccharomyces cerevisiae. Yeast, 26(10), 545–551. https://doi.org/10.1002/yea.1705
  • S. cerevisiae genome, chromosome XVI, Ty1 LTR region. GenBank: CP046096.1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]