Difference between revisions of "Part:BBa K3482011"

 
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<partinfo>BBa_K3482011 short</partinfo>
 
<partinfo>BBa_K3482011 short</partinfo>
  
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The Ptac promoter sequence here is preceeded by a coding region for lacI; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state.
  
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We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.
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===Usage and Biology===
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Revision as of 14:18, 22 October 2020


pTac promoter

The Ptac promoter sequence here is preceeded by a coding region for lacI; the function of this is to repress the promoter in it's natural state within a system. The promoter will then have to be induced, for example, by IPTG for the promoter to function in the 'ON' state.

We use this pTac promoter in our kill-switch to induce the production of the toxin (miniColicin E2 or ccdB toxin) with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]