Difference between revisions of "Part:BBa K123001"

Revision as of 03:05, 29 October 2008

BisdB

When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 479
Illegal BamHI site found at 867
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4
Illegal AgeI site found at 1276
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1086

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K123001 short</partinfo>
-When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005.
+When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.
-Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[image:bpa.jpeg]]
+Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments.
+Figure Taken From:
+ M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 105:4:1158-1169
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