Difference between revisions of "Part:BBa K3606003:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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This part is an antibiotic coding gene cluster with leader peptide and proteins for maturation.
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We introduced it to our improved antibiotic expressing system to inhibit the growth of other bacteria in human intestine so as to enhance Nissle's competitiveness,as well as to reduce risk of illness caused by some opportunistic pathogens.
  
  
 
===Source===
 
===Source===
  
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~
+
We got the plasmid containing this part and other genes from Fudan 2019
  
 
===References===
 
===References===

Revision as of 13:14, 22 October 2020


mcbABCD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
    Illegal NgoMIV site found at 1959
    Illegal AgeI site found at 2131
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is an antibiotic coding gene cluster with leader peptide and proteins for maturation. We introduced it to our improved antibiotic expressing system to inhibit the growth of other bacteria in human intestine so as to enhance Nissle's competitiveness,as well as to reduce risk of illness caused by some opportunistic pathogens.


Source

We got the plasmid containing this part and other genes from Fudan 2019

References