Difference between revisions of "Part:BBa K3328026"
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===Design=== | ===Design=== | ||
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on. | ||
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| + | https://2020.igem.org/wiki/images/thumb/8/87/T--OUC-China--design_lunbo_nimply.jpg/799px-T--OUC-China--design_lunbo_nimply.jpg | ||
===Result=== | ===Result=== | ||
Revision as of 12:43, 22 October 2020
triggers of NIMPLY gate (NIMPLY2)
two input trigger of NIMPLY2 logic gate to perform the truth table
Design
In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.
Result
The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 110
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 107
Illegal XhoI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 110
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 110
- 1000COMPATIBLE WITH RFC[1000]